The inextricable axis of targeted diagnostic imaging and therapy: An immunological natural history approach.
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The inextricable axis of targeted diagnostic imaging and therapy: An immunological natural history approach.
In considering the challenges for clinical imaging approaches, we are faced with a choice that is sometimes influenced by ideas rather dogmatic about what is better or worse technology to achieve the most useful diagnostic images of the patient. For example, PET or SPECT are most useful in imaging of any particular disease spread? Dictatorial approach would choose PET, all other things being equal.
But it is a totalitarian attitude towards imaging the selection is still valid? In the face of receptor targeted new SPECT agents we must consider the remarkable specificity and sensitivity of these agents. (99m) Tc-Tilmanocept is one of the newest of these agents are now approved to guide the sentinel node biopsy (SLNB) in several solid tumors. Tilmanocept has a Kd of 3 × 10 (-11) M, and specificity for CD206 receptor unlike other agents to date.
This coupled with the facts, that the macrophage specific related diseases express this receptor (receptor 100-150000), that the receptor has multiple binding sites for tilmanocept (> 2 sites per receptor) and that this receptor is recycled every 15 minutes to tie more tilmanocept (acts as an intracellular “drug compiler” of tilmanocept into vesicles non-degraded), give serious pause as to how we choose our approach to diagnostic imaging. Clinically, the SLN size varies greatly, some, anatomy, under resolution SPECT machine. However, with tilmanocept targeting, the SLN is very visible with stable macrophages obtained adequate (99m) Tc-tilmanocept statistical calculation, as the ratio of target-to-background can compensate for the high spatial resolution blurred.
Importantly, may be targeted imaging agents per se, more like tilmanocept, which can significantly shrink any perceived chasm between imaging technology and diagnostic considerations anchor and specificity in targeting agents than any dogma linger about the hardware as a basis for imaging approaches. Outside the application of imaging elements of these agents is their evolution to therapeutic agents as well, and even the neo-logical field of theranostics. Tilmanocept characteristics such agents that exploit the natural history of the disease with a very high specificity is hope for the future diagnosis and patient-centered disease and therapy.
The inextricable axis of targeted diagnostic imaging and therapy: An immunological natural history approach. The inextricable axis of targeted diagnostic imaging and therapy: An immunological natural history approach.
Toward the Development of the Next Generation of Rapid Diagnostic Test: Synthesis Glycophosphatidylinositol (GPI) Analog Plasmodium falciparum and immunological characterization.
A large number of proteins in the malaria parasite that is anchored using glycophosphatidylinositols (GPIs) with a lipid tail. This GPIs GPIs are structurally different from humans.
Plasmodium falciparum GPIs have been considered as potential vaccine candidates because this molecule is involved in inducing the inflammatory response in human hosts, and response to anti-GPI antibodies naturally been shown to be associated with protection against severe disease. GPIs can also be considered as a target for rapid diagnostic tests. Because the original GPIs isolation in large quantities challenging, developing a synthetic GPI molecules can facilitate further exploration of GPI molecules for diagnosis.
Here, we report the synthesis and characterization of the immunology of malaria GPI specific analog panel. A total of three analog chemically synthesized GPI and carrier protein conjugation to immunize and produce antibodies in rabbits. Rabbit immune sera showed reactivity with synthetic and GPIs GPIs original taken from P. falciparum parasite, as determined by Luminex and ELISA methods.
