Immunological characterization of monoclonal antibodies used in rapid influenza diagnostic test for detection of the 2009 pandemic influenza A(H1N1)pdm09 infection.

Since 2009 pandemic, monoclonal antibodies (mAbs) for rapid influenza diagnostic tests (RIDT) have been developed for specific diagnosis of pandemic virus infection. Most mAbs poorly marked because of the urgency during a pandemic. Further characterization of mAbs to RIDTs would be beneficial to understand the immunological properties of the pandemic virus and take advantage of mAbs for other research purposes.

In this study, it was confirmed that the two mAbs (I38 and D383) in RIDT diagnostics capable of detecting viruses H1N1pdm09 H1N1pdm09 through enzyme-linked immunosorbent assay (ELISA) and immunofluorescence test (IFA). Also, two mAbs exhibited reactivity hemagglutinins (HA) from both H1N1pdm09 and the 1918 H1N1 virus; therefore, RIDT using mAbs can detect HA of H1N1pdm09 and also the 1918 H1N1 like strains.

In an extension of our previous study, the epitope (Sa sites antigenic and interface areas of F ‘and subdomain esterase vestigial on the domain HA1 of the HA of H1N1pdm09) recognized by mAbs amplified in depth by IFA with escape-mutant mAbs and mapping epitopes the crystal structure of the human H1N1 virus HA. Collectively, these results suggest that the mAbs to RIDT may be suitable for use in studying the properties of immunology of viral H1N1pdm09 and that Sa sites antigenic and interfacial area between F ‘and esterase vestigial subdomains on influenza virus HA recognized by mAbs that immunologically conserved regions between H1N1pdm09 and 1918 H1N1.


Determination of serum autoantibodies against antigens of complex liver using enzyme immunoassay and immunoblot allows for increased frequency identification overlap syndrome during autoimmune liver disorders and early diagnostics, which has clinics, diagnostic and prognostic great importance. The level of autoantibodies overlap combining with biochemical indices and with disease activity and the intensity of the autoimmune process during overlap syndrome of primary biliary cirrhosis / autoimmune hepatitis (PBC / AIH).

 Immunological characterization of monoclonal antibodies used in rapid influenza diagnostic test for detection of the 2009 pandemic influenza A(H1N1)pdm09 infection.
Immunological characterization of monoclonal antibodies used in rapid influenza diagnostic test for detection of the 2009 pandemic influenza A(H1N1)pdm09 infection.

when immunological and molecular tools for leptospirosis: diagnostics, vaccine design, and biomarkers to predict the severity.

Leptospirosis is a zoonotic disease spirochaetal endemic in many tropical countries. Basic research on leptospirosis is not as strong as other tropical infections such as malaria. However, it is a deadly infection that can affect many vital organs in severe forms, causing multi-organ dysfunction syndrome and death. There are many gaps in knowledge about the pathophysiology of leptospirosis and host immune role in causing symptoms. This blocking an important step in combating the disease, such as developing potential vaccines.

Another major problem with leptospirosis is the lack of easy to carry, accurate diagnostic tests. Many doctors in resource-limited settings resorts for clinical assessment in the diagnosis of leptospirosis. This is unfortunate, because many other diseases such as dengue fever, hantavirus, rickettsial infection, and even severe bacterial sepsis, leptospirosis can emulate. Another interesting issue is the prediction of disease severity at the beginning of the disease.

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IK00201 96 tests
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Description: S-adenosylmethionine ELISA kit

SAH ELISA

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Description: S-adenosylhomocysteine ELISA kit

Oxycodone ELISA

EK7130 96wells/kit
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AGC ELISA Kit| Rabbit Aggrecan ELISA Kit

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CAM ELISA Kit| Rabbit Calmodulin ELISA Kit

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EPO ELISA Kit| Rabbit Erythropoietin ELISA Kit

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GS ELISA Kit| Rabbit Gelsolin ELISA Kit

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HP ELISA Kit| Rabbit Haptoglobin ELISA Kit

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INS ELISA Kit| Rabbit Insulin ELISA Kit

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LEP ELISA Kit| Rabbit Leptin ELISA Kit

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MYO ELISA Kit| Rabbit Myoglobin ELISA Kit

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PRL ELISA Kit| Rabbit Prolactin ELISA Kit

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REN ELISA Kit| Rabbit Renin ELISA Kit

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RETN ELISA Kit| Rabbit Resistin ELISA Kit

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TM ELISA Kit| Rabbit Thrombomodulin ELISA Kit

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OT ELISA Kit| Rabbit Oxytocin ELISA Kit

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Hcrt ELISA Kit| Rat Orexin ELISA Kit

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PI ELISA Kit| Rat Proinsulin ELISA Kit

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Cck ELISA Kit| Rat Cholecystokinin ELISA Kit

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ENK ELISA Kit| Rat enkephalin ELISA Kit

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GRN ELISA Kit| Rat Granulin ELISA Kit

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PRGN ELISA Kit| Rat progranulin ELISA Kit

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TPS ELISA Kit| Rat Tryptase ELISA Kit

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Pnoc ELISA Kit| Rat Prepronociceptin ELISA Kit

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CORT ELISA Kit| Rat Corticosterone ELISA Kit

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Cort ELISA Kit| Rat Cortistatin ELISA Kit

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FN ELISA Kit| Rat Fibronectin ELISA Kit

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LN ELISA Kit| Rat Laminin ELISA Kit

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Lum ELISA Kit| Rat Lumican ELISA Kit

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Prl ELISA Kit| Rat Prolactin ELISA Kit

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Lep ELISA Kit| Rat Leptin ELISA Kit

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MPO ELISA Kit| Rat Myeloperoxidase ELISA Kit

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Sost ELISA Kit| Rat Sclerostin ELISA Kit

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Epo ELISA Kit| Rat Erythropoietin ELISA Kit

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Fst ELISA Kit| Rat Follistatin ELISA Kit

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TPO ELISA Kit| Rat Thrombopoietin ELISA Kit

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Clu ELISA Kit| Rat Clusterin ELISA Kit

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Cat ELISA Kit| Rat Catalase ELISA Kit

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Tg ELISA Kit| Rat Thyroglobulin ELISA Kit

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Nesfatin ELISA Kit| Rat Nesfatin ELISA Kit

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Nrn1 ELISA Kit| Rat Neuritin ELISA Kit

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Sst ELISA Kit| Rat Somatostatin ELISA Kit

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Gck ELISA Kit| Rat Glucokinase ELISA Kit

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Ttr ELISA Kit| Rat Transthyretin ELISA Kit

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Agt ELISA Kit| Rat Angiotensinogen ELISA Kit

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Hp ELISA Kit| Rat Haptoglobin ELISA Kit

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Trh ELISA Kit| Rat Prothyroliberin ELISA Kit

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Scgb1a1 ELISA Kit| Rat Uteroglobin ELISA Kit

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REN ELISA Kit| Rat Renin ELISA Kit

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Tnmd ELISA Kit| Rat Tenomodulin ELISA Kit

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Sct ELISA Kit| Rat Secretin ELISA Kit

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Calb1 ELISA Kit| Rat Calbindin ELISA Kit

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Rnls ELISA Kit| Rat Renalase ELISA Kit

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Ache ELISA Kit| Rat Acetylcholinesterase ELISA Kit

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GT ELISA Kit| Rat Gastrin ELISA Kit

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Plg ELISA Kit| Rat Plasminogen ELISA Kit

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Gc ELISA Kit| Rat Glucagon ELISA Kit

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Eln ELISA Kit| Rat Elastin ELISA Kit

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Mme ELISA Kit| Rat Neprilysin ELISA Kit

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Nes ELISA Kit| Rat Nestin ELISA Kit

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Thrombomodulin ELISA Kit| Rat Thrombomodulin ELISA Kit

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AD ELISA Kit| Rat Adropin ELISA Kit

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Estrone ELISA Kit| Rat Estrone ELISA Kit

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E2 ELISA Kit| Rat Estradiol ELISA Kit

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HCY ELISA Kit| Rat Homocysteine ELISA Kit

EF018158 96 Tests
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GAGs ELISA Kit| Rat Glycosaminoglycan ELISA Kit

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DAG ELISA Kit| Rat Diacylglycerol ELISA Kit

EF018164 96 Tests
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MTL5 ELISA Kit| Rat Tesmin ELISA Kit

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Hyp ELISA Kit| Rat Hydroxyproline ELISA Kit

EF018211 96 Tests
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Adipolin ELISA Kit| Rat FAM132A ELISA Kit

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Calprotectin ELISA Kit| Rat Calprotectin ELISA Kit

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Ahi1 ELISA Kit| Rat Jouberin ELISA Kit

EF018267 96 Tests
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Ahcy ELISA Kit| Rat Adenosylhomocysteinase ELISA Kit

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Afm ELISA Kit| Rat Afamin ELISA Kit

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Atrn ELISA Kit| Rat Attractin ELISA Kit

EF018356 96 Tests
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Bsnd ELISA Kit| Rat Barttin ELISA Kit

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Bfsp1 ELISA Kit| Rat Filensin ELISA Kit

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Bscl2 ELISA Kit| Rat Seipin ELISA Kit

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Btd ELISA Kit| Rat Biotinidase ELISA Kit

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Ccin ELISA Kit| Rat Calicin ELISA Kit

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Cdca8 ELISA Kit| Rat Borealin ELISA Kit

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Cntln ELISA Kit| Rat Centlein ELISA Kit

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Chad ELISA Kit| Rat Chondroadherin ELISA Kit

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Cygb ELISA Kit| Rat Cytoglobin ELISA Kit

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Dfnb31 ELISA Kit| Rat Whirlin ELISA Kit

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Dstn ELISA Kit| Rat Destrin ELISA Kit

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Dixdc1 ELISA Kit| Rat Dixin ELISA Kit

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Dbn1 ELISA Kit| Rat Drebrin ELISA Kit

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Dym ELISA Kit| Rat Dymeclin ELISA Kit

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Dtnbp1 ELISA Kit| Rat Dysbindin ELISA Kit

EF018621 96 Tests
EUR 689

Most patients recover from leptospirosis with only a mild febrile illness, while others have severe disease with multi-organ failure. The clinical picture is a poor predictor of the potential severity of the infection, and because the search is for a potential biomarker that may serve as an early warning for severe disease. This review concentrates on three important aspects of these neglected tropical diseases: diagnostics, vaccine development, and potential biomarkers to predict the severity of the disease.